Rachel Crossland

Rachel Crossland

Research Associate

Articles by Rachel Crossland

  • Lyophilised Trypsin for adherent cells

    Wednesday, January 17, 2024
    I have used the Trypsin from porcine pancreas lyophilized powder (Type II-S, 1,000-2,000 units/mg dry solid) during isolation of human articular chondrocytes cells from osteoarthritis patients undergoing total knee joint arthroplasty. The powder must be resuspended at the appropriate concentration ... read more
  • Assessing MMP-13 by ELISA

    Wednesday, January 17, 2024
    I have used the Human Pro-MMP-13 Quantikine ELISA Kit to assess secreted MMP-13 protein from cultured chondrocytes, according to the manufacturer's instructions at optimized dilutions (1:10). Absorbance was measured using a Microplate Reader (Thermo Labsystems Multiskan Ascent 354) at manufacturer ... read more
  • Eradicating RNases Using RNase Zap

    Wednesday, November 29, 2023
    This product is ideal for eradicating possible RNase contaminants from lab work benches and equipment. I regularly use this product on our RNA isolation benches to ensure the workspace is RNase-free. This is especially important when working with very precious RNA samples. The product is simply ... read more
  • Alcian Blue Staining to Confirm Chondrogenesis

    Wednesday, November 29, 2023
    I have used the Alcian Blue powder in my protocol to stain mesenchymal stem cells to show chondrogenic differentiation. The product performs very well, and I am able to generate publicaiton-quality images. read more
  • 100 Place Freezer Storage Boxes with Hinged Lid

    Wednesday, November 29, 2023
    These freezer storage boxes are very robust and resistant to the plastic becoming brittle upon multiple freeze-thaw cycles at low temperatures. They can be labeled with permanent marker and the labels removed with pure ethanol. The disadvantage is that when they are full or contain standard ... read more
  • Oil-red-O Staining for Lipid Vacuole Identification

    Wednesday, November 29, 2023
    I have used oil-red-o to identify lipid vacuoles within adipocytes. Briefly, adipogenic cultures were stained for 10?min at room temperature with filtered 0.3% oil-red-O solution made with oil-red-O powder in undiluted isopropanol. After washing the cells were imaged to show lipid vacuoles within ... read more
  • Culturing MSC Cells Using StemMACS™ MSC Expansion Medium

    Wednesday, November 29, 2023
    I have used the StemMACs™ MSC expansion media to culture human mesenchymal stromal cells. Once established, cells are transferred to supplemented DMEM. The established MSCs grow well and can be fully characterised for markers and their chondrogenic, adipogenic and osteogenic potential. read more
  • Toluidine Blue Staining for Chondrogenesis Identification

    Wednesday, November 29, 2023
    I have used toluidine blue to characterize chondrogenesis potential of mesenchymal stromal cells (MSC) isolated from subchondral bone (SB) of OA patients (OA-MSCs). Staining was effective and images were of publication standard. read more
  • IHC Staining for c-Myc

    Wednesday, November 29, 2023
    I have used the rabbit monoclonal antibody (clone Y69) to perform immunohistochemical staining for c-MYC expression in diffuse large B-cell lymphoma tissues (formalin fixed paraffin embedded tissue). This was in conjunction with the Roche Ventana system. read more
  • Analysis of Immune Related Cytokines Using the V-PLEX Human Cytokine 30-Plex Kit

    Tuesday, October 03, 2023
    I have used the V-PLEX Human Cytokine 30-Plex Kit to assess expression of multiple cytokines/chemokines in human serum. The kit is multiplex and allows simultaneous assessment of multiple markers in the same sample, which is ideal when sample is limited such as during clinical studies. The results ... read more
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