The TaqMan MicroRNA Reverse Transcription Kit is a simple to use kit that comes with all components required to make microRNA specific cDNA, apart from the TaqMan RT probe of choice which is purchased separately. The downside of this method is that a separate cDNA preparation must be made for every ... read more

The nCounter® miRNA Expression Panels are a fantastic product, allowing for high-sensitivity multiplexed assessment of up to 800 microRNAs. Comprehensive controls are incorporated into the assay, including negative, positive, ligation and spike ins. The product is well suited to FFPE material and I ... read more

I use the LM10 NTA for size and concentration characterisation of extracellular vesicles (EVs). This is based on nanoparticle tracking analysis. The LM10 is not the most recent model in the range, but it is a good entry level platform to give basic EV characterisation data such as size and ... read more

I have used the Region of Interest (ROI) and Background Plates (Fast 96-well) to maintain the QuantStudio 3 qRT-PCR instrument. The plates are quick to use and can be run based on the inbuilt calibration protocol included in the QuantStudio software. This effectively maintains the instrument and ... read more

I have used the BD Pharmingen™ APC Mouse Anti-Human CD81antibody to tag extracellular vesicles (EVs) for presence of CD81. This formed part of the required characterisation from the international society of extracellular vesicles, which denotes that EVs should be characterised for presence of ... read more