I have used the Epredia™ Shandon™ Kwik-Diff™ Stains to visualize cell migration through a transwell filter. Briefly, transwell filters were swabbed, and methanol fixed. Cells were then stained using the Diff-Quick kit (Fisher Scientific) followed by ethanol dehydration (50%, 70%, 100%) and migrated ... read more

I have used the Fluoromount-G™ Mounting Medium, with DAPI to visualize nuclei. This was for human chondrocytes. I followed the manufacturer's instructions and visualized the cells using EVOS M5000 168 microscope with fluorescence at 20x and 40x magnification. The images were of publication quality ... read more

I have used PLTMax as a supplement to culture human mesenchymal stromal cells (MSC) under xenon-free conditions. Briefly, MSCs were cultured in complete medium containing Dulbecco's modified eagle medium (DMEM), 100 IU/ml penicillin, 100 µg/ml streptomycin, and 2 mM L-glutamine (Sigma-Aldrich), ... read more

I used the V-PLEX Proinflammatory Panel 1 Human Kit to assess cytokines/chemokines secreted from chondrocyte conditioned media. This was based on 1:10 dilutions and following manufacturers recommendations. The lower limits of detection (LLOD) ranged between 0.01–0.89?pg/mL. read more

I have used the Aldehyde/Sulfate Latex Beads (4% w/v, 4 µm) to increase surface area in order to perform flow cytometry for specific markers related to extracellular vesicles (EVs). Specifically, the beads work excellently with antibodies targeted to CD81, CD63, and CD9, and allow the user to ... read more