Parijat Sarkar

Parijat Sarkar

Postdoc

Articles by Parijat Sarkar

  • DMEM/F12 1:1 Cell Culture Media

    Friday, December 22, 2023
    I rely on this DMEM/F12 media for culturing IMCD3 and CHO cells for ensuring robust growth and consistent results. The F12 mixture contributes additional nutrients, vitamins, and essential components that may not be present in DMEM alone. I would highly recommend this media. read more
  • Power Pack For Running Gels

    Thursday, December 21, 2023
    I use this power pack daily for running all gels (protein/DNA). There have been no failures of this instrument and it works equally well in cold rooms. I would definitely recommend this power pack. read more
  • Good Chambered Coverslip for Imaging

    Thursday, December 21, 2023
    I use these coverslip bottom chambers for live cell imaging of mammalian cells. The glass bottom is the correct thickness for using in any high NA objectives. In my experience, these chambers could be re-used for at least three rounds of experiments. Best tip: ibidi offers free samples, so you can ... read more
  • Pre-cast Gradient Gels

    Thursday, December 21, 2023
    I have been using these pre-cast gradient gels to resolve protein complexes with a range of molecular weights. I get reproducible results every time. The only thing to keep in mind is the running buffer for these gels is different (MES or MOPS SDS Running Buffer) than regular glycine buffer. I would... read more
  • Desalting Columns

    Thursday, December 21, 2023
    I use this column to desalt purified proteins and remove urea/excess salts. The biggest advantage is time saved by not doing a dialysis for this process. I have also used this coulmn for quick buffer exchange. read more
  • Spin Column for Plasmid Miniprep

    Thursday, December 21, 2023
    I regularly use these columns for DNA miniprep purposes. They are very good quality at a reasonable price. In my experience, the binding capacity of these columns is slightly better than the other columns. read more
  • QIAprep Plasmid Miniprep Kit

    Thursday, December 21, 2023
    I use the Qiagen miniprep kit to prepare plasmid DNA for multiple downstream applications. The buffers are easy to reconstitute and plasmid yield is better than alkaline lysis method. Highly recommended. read more
  • QIAGEN Plasmid Midiprep Kit

    Thursday, December 21, 2023
    I often require larger amounts of plasmid DNA for transfection in signaling assays, and therefore use the midiprep kit. I often get 200 microg plasmid from a single column which is sufficient for transfection into multiple plates. The kit comes with all the buffers and it's very easy to follow the ... read more
  • EndoFree Plasmid Maxiprep kit

    Thursday, December 21, 2023
    I work on CRISPR screens where I need to generate viruses using >500 microg plasmids. This kit has exceptional plasmid yield suitable for such experiments. The plasmid is also endotoxin-free. Sometimes, I have used this kit for low-copy plasmids when the yield in midi-prep was low. read more
  • Mammalian Cell Culture Media - RPMI 1640 Medium

    Thursday, December 21, 2023
    I have used this media to culture HeLa cells. You need to add serum at a final concentration of 10%. This cell culture media offers great pH stability over 3-4 days. I highly recommend this media. It also exhibits remarkable consistency over different batches. read more
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