Parijat Sarkar

Parijat Sarkar

Postdoc

Articles by Parijat Sarkar

  • PBS Packets

    Wednesday, December 20, 2023
    I use these PBS powder to reconstitute into 5l water to make 1X PBS pH 7.4 The advantage of this powder is you don't have to adjust the pH of the buffer. For cell culture related work, I do recommend to filter sterilize the buffer or autoclave the solution after reconstituting. Although the packets ... read more
  • 96-Well Plate for Imaging and Fluorescence Assays

    Wednesday, December 20, 2023
    I have used the glass bottom plates for both confocal imaging and FRET assay using a plate reader. The plates are sterile so we can directly use them for plating cells. They are also much cheaper compared to other brands. All the wells are in the same plane, which allows minimum focus adjustment ... read more
  • Chamber for Confocal Imaging

    Tuesday, December 19, 2023
    I used these chambers for live cell confocal imaging of mammalian cells. These chambers have a cover glass bottom, which is essential for high-resolution microscopy. The advantages of these chambers are (1) they are sterile, making them suitable for long term cell imaging, (2) they are available in ... read more
  • Sterile Cuvettes for Electroporation

    Tuesday, December 19, 2023
    I used these cuvettes for electroporation of DNA into various mammalian cells (CHO, NIH3T3 and U2OS). The cuvettes are sold as individually wrapped and sterile, therefore minimizes chances of contamination. I have always got good transfection efficiency with these cuvettes, and therefore highly ... read more
  • Reliable PCR Tubes

    Tuesday, December 19, 2023
    I regularly clone genes by PCR, Gibson assembly and use these PCR tubes. They are melt-proof and are easy to cap on. The strip type tubes are very useful when setting-up multiple reactions. I highly recommend this product since they are cheaper and more reliable than other PCR tubes. read more
  • PCR Machine with Three Blocks

    Thursday, December 07, 2023
    The Biometra Trio PCR machine with three blocks offers a versatile solution for simultaneous processing of multiple samples. Its tri-block design enhances throughput efficiency, allowing for parallel execution of PCR reactions. The machine demonstrates precise temperature control across all three ... read more
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