Parijat Sarkar

Parijat Sarkar

Postdoc

Articles by Parijat Sarkar

  • RNA Microprep Kit

    Wednesday, January 03, 2024
    I have used this kit to isolate total RNA from mammalian cells. Compared to the iso-propanol precipitation method, the kit offers a really quick way of isolating high-quality (as assessed by RNA integrity number and 260/280 ratio) RNA. It also uses additional DNaseI treatment that helps to get rid ... read more
  • Easy to Use Gel DNA Recovery Kit

    Wednesday, January 03, 2024
    I use this kit regularly to clean up PCR products from agarose gel. The kit has very few components and therefore offers a rapid method for purifying DNA. The columns provided in this kit work with very low elution volume which helps me to concentrate the DNA sample for further cloning steps. I ... read more
  • Column for Plasmid Maxiprep

    Wednesday, January 03, 2024
    I have used these columns for plasmid Maxiprep with homemade buffers. These columns are great for obtaining high-yield DNA from 150-250 ml cultures. Please make sure to load clarified supernatant, otherwise, the column gets clogged with protein precipitates and the flow rate reduces significantly. read more
  • RNA From Any Tough-to-Lyse Sample

    Wednesday, January 03, 2024
    I use this kit to isolate RNA from Yeasts. In my hand, the kit works beautifully and I always get enough RNA for downstream assays like RT-PCR. The other advantage of this kit is it does not use phenol/chloroform/trizol and therefore could be used on bench. read more
  • PCR Purification Kit

    Wednesday, January 03, 2024
    I have used this kit for directly purifying PCR products without running in agarose gels. This saves a lot of time when you have standardized your PCR reactions. This kit could be also used for solubilized gels. read more
  • DNA Mini Kit for Tissue Samples

    Wednesday, January 03, 2024
    I have used this kit to isolate genomic DNA from cells and tissues. This has immensely helped me to screen mutant cell lines for integration for endogenous mutations. I would highly recommend this kit for its ease of use and quick results. read more
  • Magnetic Separation Rack For NGS Preparation Workflow

    Wednesday, January 03, 2024
    I used this kit for next generation sequencing library preparation workflows for magnetic bead-based purification. This is really helpful for quick washes and elution, rather than a centrifuge and spin step. This rack works with 0.2 ml PCR tubes, which is ideal for library preparations. read more
  • Syringe Filters for Sterilization

    Wednesday, December 27, 2023
    For critical cell culture applications, I prioritize solution sterility using these syringe filters. Their compatibility with various solvents and easy fitting into standard syringes make them indispensable for precise and reliable filtration. The only disadvantage is when you are working with very ... read more
  • Mammalian Cell Culture Media

    Friday, December 22, 2023
    I highly endorse this media for cultivating Hap1 cells. I have found excellent pH stability for 3-4 days, and its remarkable consistency across various batches underscores its reliability, making it a top choice for consistent and reproducible experiments. You have to add serum and antibiotics to ... read more
  • DMEM High Glucose Cell Culture Media

    Friday, December 22, 2023
    My preferred choice for cultivating HEK293 cells is this medium. However, we need to supplement it with antibiotics (penicillin and streptomycin), Glutamine, sodium pyruvate, non essential amino acids and bovine serum. Overall, this is a good 1X mixture if you want reproducible cell culture results. read more
Page

Review Laboratory Products

Review the laboratory equipment you use and receive an Amazon gift card!

Submit a Review