Parijat Sarkar

Parijat Sarkar

Postdoc

Articles by Parijat Sarkar

  • Buffer for Immunofluorescent Staining of Nuclear Proteins

    Tuesday, January 23, 2024
    I used this kit to fix and label a nuclear protein (transcription factor). The permeabilization buffer provided with the kit allows efficient nuclear membrane permeabilization which allows rapid staining of nuclear proteins. I find this kit very easy to use and the whole protocol takes roughly 2.5 ... read more
  • Trizol Reagent for Effective RNA Isolation

    Tuesday, January 23, 2024
    I use the Trizol reagent for RNA isolation from mammalian cells. I always get high yield of RNA for my RT-PCR experiments when I use this product. I have also used this reagent for preparing RNA for RNAseq experiments and the RNA integrity numbers were acceptable. I would highly recommend this ... read more
  • Mini Spin Columns for DNA Isolation

    Tuesday, January 23, 2024
    I have used these columns for plasmid mini-preps. I always get >20µg yield from 5 ml overnight cultures. The columns can carry approximately 900 ul solutions which is perfect for DNA mini-preps. Although this column is routinely used in microcentrifuges, I have used the columns in vacuum manifolds ... read more
  • Yeast Protein Extraction Reagent

    Tuesday, January 23, 2024
    I use this extraction reagent for a range of downstream applications, including Western blotting, enzyme assays, and protein analysis. Its efficiency in lysing yeast cells is great, allowing the extraction of total proteins with a high yield and minimal degradation. The rapid (15-minute incubation) ... read more
  • Master Mix for qPCR

    Tuesday, January 23, 2024
    I have found the PowerTrack SYBR Green Master Mix to be a useful alternative in my real-time PCR experiments. The inclusion of an inert blue dye enhances visibility without interfering with the PCR process, ensuring no error in my pipetting, especially when using multiple wells in a 384-well plate. ... read more
  • BCA Protein Assay Kit

    Tuesday, January 23, 2024
    For measuring protein concentration in cell lysates, I use the BCA kit. The BCA kit has proven to be an indispensable tool in my Western blot loading for accurate protein quantification. The kit's sensitivity ensures consistent and reproducible results, making it an essential asset for any ... read more
  • Excellent Thermal Cycler

    Wednesday, January 17, 2024
    I use this thermal cycler for setting up PCR reactions. The programming functions are very intuitive and easy to operate. This machine gives me reproducible runs and would highly recommend it. If you want to prolong the life of this machine, please set the hold temperature to 12 degrees. read more
  • Trypsin for Cell Dissociation

    Wednesday, January 03, 2024
    I use this trypsin-EDTA mixture for dissociating cells. This works with essentially any cell type. The 0.05% trypsin concentration strikes a balance between effective cell dissociation and maintaining cell viability. Overall, this sterile trypsin-EDTA solution is a reliable and user-friendly reagent... read more
  • Real-Time PCR System

    Wednesday, January 03, 2024
    I use the QuantStudio 5 Real-Time PCR System regularly, and its precision and user-friendly interface make it an indispensable tool for my qPCR experiments. The system's robust performance consistently delivers accurate and reproducible results. I have generally used this with SYBR green reagent, ... read more
  • Plasmid Miniprep Kit

    Wednesday, January 03, 2024
    I use the ZymoPURE Plasmid Miniprep Kit routinely for plasmid purification, and its streamlined protocol and high yield consistently give endo-toxin-free pure DNA with minimal time. The kit's efficiency and reliability have become instrumental in my molecular biology workflow. read more
Page

Review Laboratory Products

Review the laboratory equipment you use and receive an Amazon gift card!

Submit a Review