Parijat Sarkar

Parijat Sarkar

Postdoc

Articles by Parijat Sarkar

  • Poly(A) mRNA Magnetic Isolation Module

    Thursday, January 25, 2024
    I have used the NEBNext Poly(A) mRNA Magnetic Isolation Module for enrichment of mRNA and separation RNA isolation, and it has been a time-saver. This module simplifies the extraction of intact poly(A)+ RNA, perfect for library preparation and sequencing. I find the magnetic separation technology ... read more
  • Sodium Pyruvate for Cell Culture

    Thursday, January 25, 2024
    I have been using sodium pyruvate in our cell culture DMEM media as an additional carbon source alongside glucose. While not essential for all cell cultures, it's commonly added as cells naturally produce it in the glycolysis pathway. I have found that when cells are accustomed to medium ... read more
  • Great Plate Reader

    Thursday, January 25, 2024
    I am very satisfied with the the CLARIOstar Plus multi-mode plate reader. It has monochromators, filters, and a spectrometer. Its unparalleled sensitivity, combined with enhanced Dynamic Range technology, makes it the optimal choice for my experiments. The fully-flexible wavelengths and bandwidths ... read more
  • Good Control for Bromodomain Inhibitor

    Thursday, January 25, 2024
    I have been using small molecules and inhibitors from Cayman Chemicals. I used -JQ1 as a bromodomain inhibitor isomer in my experiments. It functions as a control, particularly in assays involving bromodomain inhibition. I recommend -JQ1 from Cayman Chemicals for researchers looking for a effective ... read more
  • Automated Cell Counter

    Thursday, January 25, 2024
    I have utilized the Scepter 3.0 Cell Counter for rapid cell counts, and I recommend it for its user-friendly handheld format and efficiency. The on-screen instructions are very easy to follow, and it guides through the sampling process, providing data on cell concentration, average diameter, average... read more
  • Amino Acid Supplement for Media

    Thursday, January 25, 2024
    I have used MEM Non-Essential Amino Acids as a crucial supplement in my cell culture medium, and I highly recommend it for enhancing both cell growth and viability. The solution is sterile and can be added directly to the media. This has provided robust growth and maintained the health of my cell ... read more
  • Master Mix for Reverse Transcription

    Thursday, January 25, 2024
    I have utilized the iScript Reverse Transcription Supermix for cDNA synthesis of RT-qPCR reactions. This supermix significantly enhances reproducibility and works well with dilute RNA samples as well. The protocol for cDNA synthesis is very simple and always ensures accurate gene expression analysis... read more
  • Master Mix for qPCR

    Thursday, January 25, 2024
    I have used this Master Mix for efficient quantitative PCR. Since this master mix has a low ROX concentration and is prepared at 2x, it enables robust quantitative PCR. In my experience, this is the reliable and most affordable choice among various qPCR master mixes. I highly recommend this product. read more
  • Buffer Set for Flowcytometry-Based Assays

    Tuesday, January 23, 2024
    I used the cyto-fast buffers to stain cells for flow cytometry. The buffers offer a quick alternative for fixing and permeabilizing cells in suspension for FACS. The buffers have allowed me to obtain reproducible results every time. Keep in mind that this permeabilization is suited best for labeling... read more
  • Buffer for Immunofluorescent Staining

    Tuesday, January 23, 2024
    I use this cell staining buffer to resuspend cells for flow cytometry. I have found that cells are stable in this buffer for 2-3 hours on ice. The buffer is ready to use and could be used in immunofluorescent staining of viable or fixed single cell suspensions. The buffer has added serum which helps... read more
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