Laetitia CLOTAIRE

Laetitia CLOTAIRE

Ph.D student

Articles by Laetitia CLOTAIRE

  • Chambered Coverglass System

    Tuesday, February 13, 2024
    For my immunofluorescence experiments, I used these coverglass chambers. It's a good system to do at the same time under different conditions. You can put your cells directly on the coverslip, which allows you to save a lot of time by deleting the transfert step. Be careful because there are many ... read more
  • HA Antibody

    Thursday, January 25, 2024
    Recently, I used this HA antibody in my Western blot to label my protein of interest from a whole cell extraction. The dilution range is 0.1 -0.2 µg/mL. So far, this antibody has proven effective, but it marks quite a few non-specific proteins. For now, I recommend it but I'm looking for better. read more
  • Reagent Kit for Colorimetric Protein Dosage

    Thursday, January 25, 2024
    I have been using this DC Protein Assay to determine protein concentrations. This kit is a colorimetric assay that is easy and quick to use. In two steps you can read the absorbance of your sample. All the reagents are in the kit. The only negative point is that each product runs out at a different ... read more
  • Polypropylene Cryotubes

    Thursday, January 25, 2024
    I recommend these sterile cryotubes for freezing cell samples. The special base design allows vials to be locked into a cryogenic rack and I like to use them for the round self-standing bottom. The polypropylene material of these cryotubes can withstand temperatures of -196°C. I never had a fluid ... read more
  • Trypsin for Cell Dissociation

    Tuesday, January 23, 2024
    Usually, I detached my cells by using this sterile trypsin-EDTA mixture solution (0.05%). This works easier for HEK-293, CHO, HK-2, THP-1 cells by it’s more difficult for mesenchymal stells cells and fibroblasts. I recommend this product because its quickly effective and during cell dissociation its... read more
  • Filter for Huge Volumes

    Tuesday, January 23, 2024
    I use this sterile bottle vacuum filter (0.22 µm pores) to filter my solutions specific to cell culture. In order to avoid artifacts due to the presence of particles, I prepare 1 liter of all my solutions then I filter them with this system. It's very easy to use. It has a capacity of 150 ml, and ... read more
  • Malassez Counting Chambers

    Tuesday, January 23, 2024
    I have used the Marienfeld Superior counting chambers as counting instruments to determine the number of cells present in my samples. This allows me to configure my manipulations in order to have the best reproducibility possible. This counting chambers have two chambers (top and down). You can put ... read more
  • 100 mm TC-Treated Culture Dish

    Tuesday, January 23, 2024
    I use these Petri dishes for cell culture of all adherent cells that we are studying. Among all the sizes available, I use diameters 100 for the maintenance of my cells outside of experimental manipulations. This size is very useful for increasing the number of cells. At confluence for cells like ... read more
  • Precast Western Blot Gels

    Tuesday, January 23, 2024
    I have used these precast gels in my Western Blots experiments. In our team, we use polyacrylamide gradient gels (4% to 15%) in order to have both a low molecular weight band (5 to 60 kDa) and a high molecular weight (200 kDa) proteins band on the same gel. While loading samples, you can add a ... read more
  • Cell Maintenance - Fetal Bovine Serum

    Tuesday, January 23, 2024
    I have been using this Fetal Bovine Serum as a supplement in cell culture media such as Roswell Park Memorial Institute medium (RPMI-1640), minimum essential environment (MEM) and Dulbecco's Modified Eagles Media (DMEM) to support the growth of a variety of cell lines (HEK-293, COS-7, HK-2, PBMCs, ... read more
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