Parijat Sarkar

Parijat Sarkar

Postdoc

Articles by Parijat Sarkar

  • Serum for Cell Culture

    Wednesday, February 21, 2024
    I have used the HyClone Characterized Fetal Bovine Serum (FBS) for cell culture of a wide number of cells. The batch-to-batch variability is minimum. This serum is ideal for maintaining cell viability and growth. Highly recommended. read more
  • Dynabeads for NGS Applications

    Wednesday, February 21, 2024
    I have used the Dynabeads kilobase BINDER Kit for efficient immobilization of long, biotinylated dsDNA, leveraging its high binding capacity and fast kinetics. This kit has been giving me consistent results with high batch-to-batch reproducibility, making it suitable for diverse applications in my ... read more
  • Convenient XTT Assay Reagent

    Wednesday, February 21, 2024
    I have utilized the XTT Cell Viability Assay Kit for efficient live cell number determination in assessing cell proliferation and cytotoxicity. Compared to conventional MTT assay, its use of XTT eliminates the solubilization step needed in MTT assays, providing a rapid assay in assessing cell ... read more
  • Plasmid Midiprep Kit

    Wednesday, February 21, 2024
    I routinely use the ZymoPURE II Plasmid Midiprep kit for fast and efficient isolation of transfection-grade plasmid DNA from E. coli. With the ability to process up to 50 ml of culture, this kit has provided me significantly more plasmid DNA and reduces processing times, offering a convenient and ... read more
  • Antibiotics for Cell Culture

    Wednesday, February 21, 2024
    I have been using this 100X penicillin and streptomycin solution in my cell cultures to prevent bacterial contamination. This combination of antibiotics, are effective action against both gram-positive and gram-negative bacteria. It's a reliable addition to prevent common bacterial contaminations, ... read more
  • L-Glutamine Supplement for Cell Culture

    Wednesday, February 21, 2024
    I use L-Glutamine, an essential amino acid, as a crucial additive for cell culture. Adding 1x of it in culture media significantly enhances cell growth. This reagent has consistently performed well, ensuring our cultures without any issues of impurity or contamination. It proves convenient for in-... read more
  • Master Mix for qPCR

    Thursday, January 25, 2024
    I have used this Master Mix for efficient quantitative PCR. Since this master mix has a low ROX concentration and is prepared at 2x, it enables robust quantitative PCR. In my experience, this is the reliable and most affordable choice among various qPCR master mixes. I highly recommend this product. read more
  • Master Mix for Reverse Transcription

    Thursday, January 25, 2024
    I have utilized the iScript Reverse Transcription Supermix for cDNA synthesis of RT-qPCR reactions. This supermix significantly enhances reproducibility and works well with dilute RNA samples as well. The protocol for cDNA synthesis is very simple and always ensures accurate gene expression analysis... read more
  • Amino Acid Supplement for Media

    Thursday, January 25, 2024
    I have used MEM Non-Essential Amino Acids as a crucial supplement in my cell culture medium, and I highly recommend it for enhancing both cell growth and viability. The solution is sterile and can be added directly to the media. This has provided robust growth and maintained the health of my cell ... read more
  • Automated Cell Counter

    Thursday, January 25, 2024
    I have utilized the Scepter 3.0 Cell Counter for rapid cell counts, and I recommend it for its user-friendly handheld format and efficiency. The on-screen instructions are very easy to follow, and it guides through the sampling process, providing data on cell concentration, average diameter, average... read more
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